| Alternative title | ACE2-1 : Matériau de référence de l’enzyme de conversion de l’angiotensine 2 |
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| DOI | Resolve DOI: https://doi.org/10.4224/crm.2022.ace2-1 |
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| Author | Search for: Stocks, Bradley1ORCID identifier: https://orcid.org/0000-0002-7265-9344; Search for: Thibeault, Marie-Pier1; Search for: L'Abbé, Denis1; Search for: Stuible, Matthew1; Search for: Durocher, Yves1ORCID identifier: https://orcid.org/0000-0002-2268-4111; Search for: Melanson, Jeremy1ORCID identifier: https://orcid.org/0000-0002-2290-0101 |
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| Affiliation | - National Research Council Canada
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| Format | 3D, Material |
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| Material type | Calibration solution |
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| Subject | Canadian reference material; ACE2-1; angiotensin-converting enzyme 2 protein |
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| Abstract | ACE2-1 is a reference material (RM) designed for use in coronavirus spike protein binding assays, and for evaluating antibody-mediated inhibition of the receptor-spike interaction. The reference values for ACE2-1 are based on combined results from two orthogonal methods from data generated at the National Research Council of Canada (NRC). A unit of ACE2-1 contains 50 µL of biotinylated human angiotensin-converting enzyme 2 (ACE2) in Dulbecco's phosphate-buffered saline. |
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| Analytes | | Analyte | Quantity | Value | Expanded uncertainty | Unit | Type |
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| angiotensin-converting enzyme 2 | molar concentration | 25.3 | 2.3 | µmol/L | reference | | angiotensin-converting enzyme 2 | mass fraction | 1.9 | 0.2 | mg/g | reference | | angiotensin-converting enzyme 2 | mass concentration (21 oC) | 1.9 | 0.2 | mg/mL | reference | | angiotensin-converting enzyme 2 | molar mass | 76107 | 1 | g/mol | reference | | angiotensin-converting enzyme 2 glycoprotein | molar concentration | 25.3 | 2.3 | µmol/L | reference | | angiotensin-converting enzyme 2 glycoprotein | mass fraction | 2.1 | 0.2 | mg/g | reference | | angiotensin-converting enzyme 2 glycoprotein | mass concentration (21 oC) | 2.1 | 0.2 | mg/mL | reference | | angiotensin-converting enzyme 2 glycoprotein | molar mass | 84000 | 5000 | g/mol | reference |
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| Period of validity | - Valid from 2025-10-16 until 2031-06-30
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| Storage conditions | |
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| Publication date | 2022-02-01 |
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| Publisher | National Research Council Canada |
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| Copyright statement | - © 2022
National Research Council Canada
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| Access condition | |
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| References | View items (4)- Stocks, Bradley B., Marie-Pier Thibeault, Denis L’Abbé, Muhammad Umer, Yali Liu, Matthew Stuible, Yves Durocher, and Jeremy E. Melanson. ‘Characterization of Biotinylated Human ACE2 and SARS-CoV-2 Omicron BA.4/5 Spike Protein Reference Materials’. Analytical and Bioanalytical Chemistry 416, no. 22 (28 June 2024): 4861–72. https://doi.org/10.1007/s00216-024-05413-7.
- Colwill, Karen, Yannick Galipeau, Matthew Stuible, Christian Gervais, Corey Arnold, Bhavisha Rathod, Kento T Abe, et al. ‘A Scalable Serology Solution for Profiling Humoral Immune Responses to SARS‐CoV‐2 Infection and Vaccination’. Clinical & Translational Immunology 11, no. 3 (January 2022). https://doi.org/10.1002/cti2.1380.
- Veen, Adriaan M. H. van der. ‘Bayesian Analysis of Homogeneity Studies in the Production of Reference Materials’. Accreditation and Quality Assurance 22, no. 6 (2 November 2017): 307–19. https://doi.org/10.1007/s00769-017-1292-6.
- ‘Evaluation of Measurement Data — Guide to the Expression of Uncertainty in Measurement’, 2008. https://doi.org/10.59161/jcgm100-2008e.
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| Export citation | Export as RIS |
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| Collection | Canadian Reference Materials and Methods |
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| Record identifier | df43c24e-3213-4f26-94cd-dc273d988f6a |
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| Record created | 2022-02-21 |
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| Record modified | 2025-11-20 |
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